http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1326523-A4
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_01a96e8bbea00eeaa0b50c2a0c000dba |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01R33-465 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01R33-48 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01R33-465 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01R33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2001-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8c437c4e9287c1e5f751adf15fdff73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_824e780cca974e92e3ec148690cfd700 |
publicationDate | 2005-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1326523-A4 |
titleOfInvention | VISUALIZATION OF THE ENZYMATIC ACTIVITY |
abstract | The invention features methods of detecting enzymatic activity (e.g., in a magnetic resonance image). The methods include: (I) providing a monomeric substrate (e.g., a substrate that is polymerizable in the presence of an enzyme or as a result of an enzyme-catalyzed reaction), having the generic structure X-Y-Z, where X includes a chelator moiety having a chelated paramagnetic or supermagnetic metal atom or iron, Y includes a linker moiety (e.g., to provide a covalent or non-covalent chemical bond or bonds between X and Z), and Z includes a polymerizing moiety; (2) contacting the substrate with a target tissue, wherein the substrate undergoes polymerization to form a paramagnetic or supermagnetic polymer, the polymerization being catalyzed by an enzyme in an extracellular matrix or bound to the surfaces of cells of the target tissue; and (3) detecting an increase in relativity for the polymer relative to an equivalent amount of unpolymerized substrate. The invention also features substrate compositions. Figure (1) is a synthetic pathway used for synthesis of an enzyme-responsive, paramagnetic monomeric substrate. |
priorityDate | 2000-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1229.