| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2846e1eb7c95f81244da78ae9bf5ae09 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70539 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
1995-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b18fc30c9590e224a5c6ef00f087d13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa7a60174163d765baea7f8aa9c66120
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37130d78eea1820c19950ab583ad8772
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9145bc546f69e616f2e3c723fb639923 |
| publicationDate |
2003-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1319711-A2 |
| titleOfInvention |
Production of pharmaceutical-grade plasmid DNA |
| abstract |
The invention relates to a method for producing plasmid DNA,ncomprising the steps of: (a) lysing cells containing the plasmidnDNA to obtain a lysate; (b) treating the lysate by a means fornremoving insoluble material to obtain a solute; and (c) applyingnthe solute to differential PEG precipitations and chromatographynto purify the plasmid DNA. In other embodiments of the invention,nthe plasmid DNA is produced with GRAS reagents; the plasmid DNA isnproduced in the absence of enzymes; the plasmid DNA is produced innthe absence of organic extractants; the plasmid DNA is produced innthe absence of mutagens; the lysing, treating and applying stepsnare scalable to result in the large scale manufacture of thenplasmid DNA; and the lysing, treating and applying steps result innthe generation of pharmaceutical grade material. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005094960-A1 |
| priorityDate |
1994-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |