http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1315757-A2

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-8114
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-81
filingDate 2001-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c5ebb20499494b120b2053bb871776a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80a98d6a78994a161a49210ed0ca9bc1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7108139bb07cfe39e268a3552ee981a0
publicationDate 2003-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1315757-A2
titleOfInvention Process for the purification of epi-hne proteins
abstract The present invention concerns a process for the purification of EPI-HNE proteins, from the culture medium of a host strain for the expression of said proteins, comprising the steps of: (a) passing a derived part of the culture medium over an expanded bed of cationic exchange adsorbent or a mechanically and chemically inert micromembrane, in order to recover an eluate, (b) optionally conducting chromatographic separation of proteins, according to their hydrophobicity, on the resulting eluate, (c) passing the resulting eluate over a cationic exchange column, (d) optionally filtering the resulting medium such as to obtain a sterile filtrate, and optionally further comprising the step of (e) causing precipitation of EPI-HNE in a crystallised form and recovering the protein crystals.
priorityDate 2000-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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