http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1315757-A2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5f353af12db08b6b7836ed3789d83b8e |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-8114 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-81 |
filingDate | 2001-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c5ebb20499494b120b2053bb871776a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80a98d6a78994a161a49210ed0ca9bc1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7108139bb07cfe39e268a3552ee981a0 |
publicationDate | 2003-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1315757-A2 |
titleOfInvention | Process for the purification of epi-hne proteins |
abstract | The present invention concerns a process for the purification of EPI-HNE proteins, from the culture medium of a host strain for the expression of said proteins, comprising the steps of: (a) passing a derived part of the culture medium over an expanded bed of cationic exchange adsorbent or a mechanically and chemically inert micromembrane, in order to recover an eluate, (b) optionally conducting chromatographic separation of proteins, according to their hydrophobicity, on the resulting eluate, (c) passing the resulting eluate over a cationic exchange column, (d) optionally filtering the resulting medium such as to obtain a sterile filtrate, and optionally further comprising the step of (e) causing precipitation of EPI-HNE in a crystallised form and recovering the protein crystals. |
priorityDate | 2000-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.