| abstract |
The invention concerns novel yeast strains, methods and genetic constructs for preparing them, and their use for synthesising or modifying steroid compounds. More particularly, the invention concerns strains having reduced 20α-HSD activity, in particular by modification of the GCY1 and/or YPR1 genes. The inventive yeast strains enable to improve the efficacy of synthesis or to increase selectivity or yields of the process, as well as the quality of the final product. The inventive strains, methods and compounds are useful for researching, developing and producing products with therapeutic or prophylactic activity, in a human or an animal, in particular steroid derivatives. |