| abstract |
The invention relates to a method and to an assembly for operating an optical imaging system for detecting the characteristic values of the wavelength-dependent behavior of an illuminated specimen, especially of the emission and/or absorption behavior, preferably of the fluorescence and/or luminescence and/or phosphorescence and/or enzyme-activated light emission and/or enzyme-activated fluorescence, preferably for the purpose of operating a laser scanning microscope. According to the inventive method, the image spot information of the specimen is broken down into its spectral components in a spatially resolved and wavelength-independent manner on the detector end, and for different spectral components at least one summation is made. |