http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1302548-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_55bc2bae9f65cfaede67b62128f00e9b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2002-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2a43f97d8dc9a5e95d2bdd35e1777c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33425dc462f6674be267b9d595625795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8157cfb4c4c0ca545f62ef4be7be19c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6ca2535e404195992c5202ee2bae775 |
| publicationDate | 2003-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1302548-A1 |
| titleOfInvention | Buffer composition for hybridization of microarrays |
| abstract | A buffer composition, method and kit for hybridizing microarrays of nucleicnacids bound to an adsorbed polymer surface of a siliceous substrate provide annenvelope of conditions to hybridize nucleic acid targets, while preserving thenintactness of the adsorbed polymer surface of the array. The buffer compositionncomprises a non-chelating buffering agent, a pH between pH 6.4 and 7.5, anmonovalent cation having a monovalent cation concentration that ranges from 0.01 Mnto 2.0 M, and optionally, relatively smaller concentrations of a chelating agent and annionic surfactant. The total cation concentration of the buffer composition ranges fromnabout 0.02 M to about 2.0 M. The method comprises incubating the targets with anhybridization solution comprising the buffer composition and the siliceous substrate atna temperature between 55°C and 70°C. |
| priorityDate | 2001-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 119.