| abstract |
Disclosed is a method and apparatus fornregenerating an oxygen-binding ability by reducing thenmethemoglobin contained in hemoglobin-vesicle by lightnirradiation. This method comprises adding electronndonors such as amino acids, saccharides, alcohols,nand/or flavin derivatives in appropriate amounts to theninner aqueous phase of the hemoglobin-vesicle, applyingnlight to the vesicle when the content of methemoglobinnincreases as a result of oxidation of hemoglobin,nthereby reducing the metohemoglobin. In this manner,nthe oxygen-binding ability is recovered, therebynmaintaining the function of the hemoglobin-vesicle. |