| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff3e741d8c3082a79ba472a5a4c2bef8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-101 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate |
2001-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2006-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4903a12cd99a69496919bd211597d418 |
| publicationDate |
2006-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1294945-B1 |
| titleOfInvention |
Method for detecting cytosine methylations |
| abstract |
Detection of cytosine methylation in DNA and detecting to what extent the (A)-treated and original DNAs differ, and deducing the methylation status at least one locus in the DNA, is new. Detection of cytosine methylation in DNA comprises: (i) incubating a genomic DNA sample with 0.1-6 M bisulfite solution (A); (ii) dilution with water or aqueous solution; (iii) amplification in a polymerase reaction; and (iv) detecting to what extent the (A)-treated and original DNAs differ, and deducing the methylation status at least one locus in the DNA. Step (i) is in presence of a denaturing agent and/or solvent, also of at least one radical scavenger (I). An independent claim is also included for a kit for the process comprising (A), denaturant or solvent, (I), primers for amplification and instructions. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021013534-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102019118332-B4 |
| priorityDate |
2000-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |