http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1283901-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_286d5c0dae7ad21e9e58e736d163f9eb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 |
| filingDate | 2000-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2004-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82e3575565df758d4bcd751ee372d659 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d3dc0a5d9fd7cf78857f7bca85a5224 |
| publicationDate | 2004-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1283901-B1 |
| titleOfInvention | Method and reagent kit for determining activity of 5'-nucleotidase |
| abstract | The invention relates to a method for determining activity of 5'-nucleotidase, in which a biological sample is incubated in a manner and under conditions known per se with a nucleotide pentose monophosphate as substrate, the liberated inorganic phosphate is converted into a colored complex by treating it with ammonium molybdate and a reducing agent, color intensity is measured by a known method, and from the measured value the activity of 5'-nucle-otidase or the amount of inorganic phosphate liberated within unit time, as a figure proportional to the activity of 5'-nucleotidase, is calculated with a known calculation and/or with the aid of a calibration curve. According to the invention 5'-AMP, 5'-CMP, 5'-UMP, 5'-GMP, 5'-IMP and 5'-TMP are used as nucleotide pentose monophosphates, and measurement is performed on all of these six substrates. The invention also relates to a reagent kit for performing the above method. |
| priorityDate | 2000-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.