http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1280893-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aae6dbe01f19b543a4d00b66763cfc65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d060c20f6f6aed91a3fc99904b9af20b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2001-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c02d70cc8710d8fb80d1375d7e5bc2d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_092da20161d3d9619072f8136d02bf58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d04aa5e89e2e1db4e78de37a6981be73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b434e667e95d98781f0ab1f68db39775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_988bcd9c7fb158ab21cc2b59c8381965 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c6ec4e6a4ef348e1eb94a97581645ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6426f0aea845f974947ed05b4f710aaf |
| publicationDate | 2003-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1280893-A2 |
| titleOfInvention | Method for the production of proteins in a yeast of the genus arxula and suitable promoters |
| abstract | The invention relates to a method for the production of one or several proteins in a host cell of the yeast genus Arxula and a nucleic acid molecule, comprising a suitable promoter. The invention further relates to an expression vector, a host cell, a kit and the use thereof. The aim of the invention is a method for the production of a protein in a yeast of the genus Arxula, by means of which large amounts of protein may be obtained. Said aim is achieved by means of a method for the production of one or several proteins in a host cell of the yeast genus Arxula, comprising: (i) cloning of at least one nucleic acid, which codes for a heterologous protein in an expression vector, containing an inducible promoter from a yeast of the genus Arxula, or a promoter from a constitutively expressed gene, chosen from the ARFC3 gene and the AHSB4 gene, from a yeast of the genus Arxula, such that the cloned nucleic acid is under the transcription control of the promoter; (ii) introduction of the expression vector obtained in (i) into a host cell of the yeast genus Arxula, suitable for the production of the heterologous protein; (iii) cultivation of the host cells obtained in (ii) and, optionally, (iv) induction of the promoter; (v) harvesting the protein by a known method. |
| priorityDate | 2000-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 936.