http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1276909-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1019fb51220a23555730ab96763d96d5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-703 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2001-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e88ab5390325c028b061d13525b16da2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2970f341a92db3fc763c486fe4c9e63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8ca4f9da24720ad8395d45fd7a3cd4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63728204adc2e2693dbda5a820e33cae |
| publicationDate | 2003-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1276909-A1 |
| titleOfInvention | Method for mutation detection in hiv using pol sequencing |
| abstract | The present invention relates to a method for mutation analysis of the HIV pol gene of HIV virions comprising amplifying virion RNA or DNA via nested PCR using outer primers as represented in SEQ ID No. 1 and 2, amplifying said PCR product via nested PCR using a 5' and 3' primer chosen from the inner primers SEQ ID No. 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No. 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No. 13 to 24. The benefit of the sequences present in the invention resides in the fact that, with the aid of the oligonucleotides, the sequences of all presently known HIV subtypes and all mutations of the pol gene presently known to yield resistance towards antiretroviral therapy can be determined. The present invention also relates to kits for performing such a method as well as primers for performing the same. |
| priorityDate | 2000-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 160.