| abstract |
A method for measurement of a target substance in a sample by means ofnfluorescence, which comprises the step of carrying out the measurement in thenpresence of (1) a specific fluorescent probe which specifically reacts with the targetnsubstance to generate fluorescence, and (2) a donor which, per se, has long-livednfluorescence and is capable of inducing fluorescence resonance energy transfer to thenspecific fluorescent probe that acts as an acceptor, provided that the donor forms nonbinding to the specific fluorescent probe by means of a chemical bond; and the step ofnconverting fluorescence, wherein said fluorescence is resulted from a reaction betweennthe acceptor and the target substance, into long-lived excitation fluorescence by thenfluorescence resonance energy transfer which is induced on the acceptor. |