| abstract |
The present invention relates to improved promoters and utilizationnthereof, in particular to promoters which are improved so as not to undergonmethylation in the course of constructing transformants, and utilization thereof. n The improved promoters of the present invention are represented by thenfollowing (1) and (2):n (1) a DNA comprising the nucleotide sequences shown in SEQ ID NOS: 1 to 4; (2)na DNA comprising a nucleotide sequence consisting of the nucleotide sequencesnshown in SEQ ID NOS 1 to 4, wherein one to several nucleotides are deleted,nadded or inserted and the deleted, added or inserted sequence is free from anynconsecutive sequences represented by CG, CAG, CTG, CCG or CGG; the DNAnhaving promoter activity. n According to the present invention, the expression efficiency of anstructural gene can be enhanced even in a plant, e.g. chrysanthemum, which hasnweak expression of the structural gene by a cauliflower mosaic virus 35S promoternwhich has been considered a high expression promoter for plants. |