Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5c718ada04b08ffc251ea5c07da78fbc |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
filingDate |
2001-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a7942792567b6f492a5e9175224814a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f533ee6b9cfadbccf85492727cc63a6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb4fc3635f0c963790bde07398229b35 |
publicationDate |
2002-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-1263973-A2 |
titleOfInvention |
Dna joining method |
abstract |
The present invention provides a method to directionally clone any linear template DNA molecule into any linearized vector. The vector ends may be generated from any restriction enzyme cleavage. The method does not require a ligation step nor the use of carefully controlled conditions as is required with methods involving specific exonucleases alone. It has been determined that specific DNA polymerases are able to efficiently join one or more linear DNA molecules sharing ends with appropriate complementation. |
priorityDate |
2000-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |