| abstract |
This invention is directed to compositions and methods of screening, sequencing, and/or quantitating a nucleic acid of interest by hybridizing that nucleic acid with a set of oligonucleotide probes, which are coupled to fluorescently addressable multicolored microparticles. These microparticles are provided as a liquid array that can be positioned in predetermined wells or reaction vessels of a microtiter plate. For sequencing purposes, each such liquid array preferably comprises every possible combination of sequences for a given length of a probe. Hybridization occurs by complementary recognition of the analyte of interest with a probe. Probe, target, and/or competing molecule are tagged with a reporter molecule so that upon hybridization, the changes in fluorescence signal parameters are recorded and analyzed. |