http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1243661-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_593f1a3ba6ae19dde0c7ddf687e26640 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d75d1a4caed60a9316608ed9c231546c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 1997-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36a33a76ce02ecc8e7c8004db0e1ea1b |
| publicationDate | 2002-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1243661-A1 |
| titleOfInvention | Method for reverse transcription |
| abstract | A method for preparing a cDNA from a mRNA using a reversentranscriptase wherein reverse transcription is performed at antemperature at which the mRNA does not take a secondary structure,nfor example, at a temperature of 45°C or more. The method isnperformed, for example, using a heat-labile reversentranscriptase in the presence of a substance exhibitingnchaperone function having chaperone function such asnsaccharides. The method is performed, for example, in thenpresence of metal ions necessary for activation of the reversentranscriptase and a chelating agent for the metal ions such asna deoxynucleotide triphosphate. The method is capable ofnreverse transcription over the full length of mRNA template evennif the mRNA is a long chain mRNA and, as a result, producingna full length cDNA. |
| priorityDate | 1996-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 166.