http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1241268-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_358a329cc314c43da9d13d9d90a7db83 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2002-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6b0bc8c73cd5a1768dca357932e5674 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_749404d1ca05a97be3f2e9620e9875c7 |
| publicationDate | 2002-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1241268-A2 |
| titleOfInvention | Method for amplifying RNA |
| abstract | Poly(A) + RNA extracted from a cell or a tissue isnamplified by the following steps of:n (a) synthesizing a cDNA first strand by using RNAnextracted from the cell or the tissue as a template andna first primer having an oligo-dT and a promoternsequence, (b) joining a sequence to which a second primerncan anneal to the 3' end of the cDNA first strand, (c) synthesizing a cDNA second strand by using thencDNA first strand as a template and the second primer tonobtain a double-stranded cDNA, and (d) synthesizing RNA by using the double-strandedncDNA obtained in the step (c) and an RNA polymerasencorresponding to the promoter sequence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012150455-A1 |
| priorityDate | 2001-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 152.