http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1241267-A8
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8cf156a55ab331c54eb2daf939b73e76 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2002-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d413472b6480853787fd2eeafe8cc66e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_985e1c575f97e8fa82023315c5551857 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2630d2383e4ca4a94ea3065e652e63ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8980b39b29073416278a8eca855fa925 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce8cdd0745d867fe0a5baad26eac62f8 |
| publicationDate | 2003-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1241267-A8 |
| titleOfInvention | Enzyme activity test using fluorescently labeled oligonucleotide substrate |
| abstract | The invention relates to a method for the detection of a substance with anenzymatic activity leading to sequence specific depurination ornDepyrimidination of a nucleic acid without strand termination leads, or with ansequence specific endonuclease activity, in a sample, wherein (a) the samplen(aa) is contacted with a substrate that (i) threenNucleic acid sequence sections (A, B and C), wherein thenNucleic acid sequence section B is between sections A and C.nNucleic acid sequence sections A and C have sequences that are complementarynto each other and thus to each other under physiological conditionsnhybridize and thereby the substrate forms a hairpin structure and wherenthe nucleic acid sequence section B or the nucleic acid sections A and C.nHybridization included a recognition motif for the activity; and (ii) anHas fluorophore-quencher pair, the fluorophore covalently withnSequence section A or C is connected and the quencher is covalently linked to thenanother sequence segment (C or A) and where fluorophore andnQuenchers in the hybridization of the DNA sequences in close proximitynto each other, so that the light energy emitted by the fluorophore fromnQuencher is quenched; and (ab) if the substance is a substance with anis enzymatic activity leading to sequence specific depurination ornDepyrimidination of a nucleic acid without strand breakage leads with an agent innIs brought into contact that the substrate specifically at a nucleotide position in thenRegion of the nucleic acid sequence section B cleaves by the substancenis depurinated or depyrimidinated; and (b) based on the emission of the fluorophor specific onesnLight energy is determined whether a substance with the saidnThere is activity in the sample. The sample to be tested preferably containsnRibosome inactivating protein (RIP), in a special embodimentnMistletoe. The invention further relates to a test kit for performing thenMethod which uses the substrate specifically labeled with fluorophore and quencher,nthe chemical cleavage reagents and, if applicable, those for the reactionsncontains prepared reaction vessels. |
| priorityDate | 2001-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.