Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_662717c94dd93edb84c2df5ba0d5e45c |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2220-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2220-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-528 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2220-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-525 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-28042 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-261 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-281 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-327 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-28014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-321 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-3246 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-285 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-287 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-366 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-3064 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-281 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-26 |
filingDate |
2001-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15826674e5d87183847ea2dc909c46cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b16b7e93705ca6e251cf71bb2c07da1b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_edd08c0943f84aeae8f4dba1a54426d8 |
publicationDate |
2002-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-1235852-A2 |
titleOfInvention |
Method and apparatus for separating polynucleotides using monolithic capillary columns |
abstract |
Methods and devices based on capillary monolithic columns, preferably consisting of an underivatized poly(styrene-divinylbenzene) monolith, for separating a mixture of polynucleotides by ion pair-reverse phase-high performance chromatography (IP-RP-HPLC). In various aspects of the method and device the monolith is characterized by one or more of the following: the monolith is contained within a capillary tube; the monolith is immobilized by covalent attachment at the inner wall of the tube; the tube is devoid of retaining frits; the monolith is characterized by having above 10,000 theoretical plates per meter and preferably above 200,000 theoretical plates per meter; the method uses a mobile phase which is devoid of EDTA; the monolith has a surface morphology that is rugulose or brush-like; the chromatographic surfaces of the monolith are non-porous; the monolith has channels sufficiently large for convective flow of the mobile phase; the monolith is formed from a polymerization mixture including underivatized styrene, a crosslinking agent, and a porogen, wherein the porogen includes tetrahydrofuran. The monolith can be incorporated into a miniaturized chromatography system which can be coupled to a mass spectrometer for on-line separation and mass determination of single- or double-stranded polynucleotides. |
priorityDate |
2000-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |