http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1232283-B1

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filingDate 2000-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2006-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8ae48fd4ac74f8f67354f6d3fd880d8
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publicationDate 2006-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1232283-B1
titleOfInvention Immuno-real-time PCR using a chimeric DNA marker for amplification
abstract The invention concerns a method using PCR, in particular immuno-PCR, for detecting microbiological entities and in particular elements or target molecules. The detection method comprises a step which consists in fixing an entity A, more particularly an antigen, directly or via a capture agent, for example an antibody, to one or several surface(s) inside the receptacle(s) of a real time PCR measuring apparatus and a step which consists in associating with said element A a coupling system with a nucleic acid, for example a DNA marker, a step which consists in amplifying the marker nucleic acid. The successive measurements are carried out during the phase amplifying the marker nucleic acid, in the presence of a capture agent and other optional intermediate fixing agents. The detection method is quantitative and can be used in particular for prion polypeptide assay.
priorityDate 1999-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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