| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7ce3af5efbbdf0d4388b857c317b4849
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_033b1684a9d95fd8f6f480ce03de2ad9 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00707
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00596
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0061
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00659
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00689 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2000-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4957e4ce438b0a7d8a7e42e90a4f59bc |
| publicationDate |
2002-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1230383-A2 |
| titleOfInvention |
Quantitative assay for expression of genes in microarray |
| abstract |
A method has been developed for detection of gene expression or hybridization in microarrays, for example, in combinatorial libraries where quantities are very small and spots located very closely, resulting in uncomfortable situations where intense reaction can spill over into the adjacent spots, and therefore obscure the accuracy of the reaction of the neighboring sites. The assay uses a digoxigenin enzyme assay for detection. A method for enhancing the reliability of analysis of expression of DNA in microarray formats has also been developed, using software analysis that normalizes the spots. This process uses deformable template techniques to quantify large-scale array data automatically, despite possible spatial distortion of the arrays. Each node in the deformable template represents a gene spot, and iterates according to the gradient descent rule, which minimizes an energy function combining data mismatch energy and template deformation energy. |
| priorityDate |
1999-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |