abstract |
A biochemically defined culture medium for culturing engineered Chinese hamster ovary (CHO) cellnlines, which is essentially free from protein, lipid and carbohydrate isolated from an animal source,ncomprising water, an osmolality regulalor, a buffer, an energy source, amino acids including L-glutamine,nan inorganic or recombinant iron source, and a synthetic or recombinant growth factor, andnoptionally non-ferrous metal ions vitamins and cofactors; also cells adapted to grow in such a culturenmedium. |