Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7975d9430e7f133f514b58796570fc22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_408700e1d322c39dda567d4fd11786d9 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6467 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
filingDate |
2000-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51b6eb574d7d171e61ea3a249e012f73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b95da10ac61c80cf30ea310d1d827fe3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a19a02004c5d1a70d53f38da3b81e199 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10ccaed1f78f4cf3d65bb0b89facd31e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e366bbbbd4a56ec0fd0599b5bc887d3c |
publicationDate |
2002-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-1214428-A1 |
titleOfInvention |
Method for producing active serine proteases and inactive variants |
abstract |
The invention relates to a method for producing biologically active serine proteases, isolated serine protease domains and their amino-acid variants in a prokaryotic host. The method is characterised by an N-terminal addition of a helper sequence consisting of a dipeptide which is suitable for degradation by a dipeptidyl amino peptidase, by the expression of the serine protease(s) and/or their fragments containing the N-terminal dipeptide helper sequence, optionally as inclusion bodies, and by the renaturation of the expressed proteins and the activation of the serine protease(s) and/or serine protease domains by splitting-off the helper sequence using an exopeptidase. |
priorityDate |
1999-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |