| abstract |
Provided are an alkaline protease wherein an amino acid residue at (a)nposition 84, (b) position 104, (c) position 256 or (d) position 369 of SEQ IDnNO:1 or at a position corresponding thereto has been deleted or selectednfrom: at position (a): an arginine residue, at position (b): a proline residue, atnposition (c): an alanine, serine, glutamine, valine, leucine, asparagine,nglutamic acid or aspartic acid residue, and at position (d): an aspartic acidnresidue; an alkaline protease wherein an amino acid residue at (e) position 66nor 264, (f) position 57, each of 101 to 106, 136, 193 or 342, (g) position 46 orn205, (h) position 54, 119, 138, 148 or 195, (i) position 247, (j) position 124, (k)nposition 107 or (I) position 257 has been deleted or selected from: at positionn(e): a glutamine, aspartic acid or like residue, at position (f): a lysine, serine ornlike residue, at position (g): a tyrosine or tryptophan residue, at position (h): antryptophan, phenylalanine or like residue, at position (i): a tryptophan,nphenylalanine or like residue, at position (j): an alanine or lysine residue, atnposition (k): a lysine, arginine or like residue, and at position (I): a valine ornisoleucine residue; a gene encoding the alkaline protease; a recombinantnvector containing the gene, a transformant containing the recombinant vector;nand a detergent composition containing the alkaline protease. nThe present invention makes it possible to provide alkaline proteases havingnactivity even in the presence of a high concentration of fatty acids, havingnhigh specific activity and detergency and being useful as an enzyme to benincorporated in a detergent. |