| abstract |
A method of linking a first molecule M1-NH2 with a second molecule M2-OH comprising reaction of a compound of the formula (I): M1-NH-CO-A-CO-SR1 wherein M1 is the residue of a molecule bearing an amino group, A is an alkylene or arylene group, R1 is alkyl or aryl, with a compound of formula (II), M2 is the residue of a molecule bearing a hydroxy group wherein B is a linker, D is C¿1-4? alkylene group or C3-12 arylene group, R?2¿ is hydrogen or a thiol protecting group. In addition, this invention relates to conjugate products of the coupling reaction, reagents for modifying M1-NH2 and M2-OH, and kits comprising these reagents. |