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filingDate 2000-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4903a12cd99a69496919bd211597d418
publicationDate 2002-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1204765-A2
titleOfInvention Method for relative quantization of methylation of cytosin-type bases in dna samples
abstract A method is described for the relative quantification of the methylation of cytosine bases in DNA samples, wherein the following method steps are conducted: a) a genomic DNA sample is chemically converted with a reagent, wherein 5-methylcytosine and cytosine react differently and show a different base pairing behavior in the DNA duplex after the reaction; b) the DNA sample is amplified, whereby a fluorescently labeled dCTP or dGTP derivative is added; c) the amplified products are separated spatially from each other; and d) the fluorescence of the separated amplified products is measured quantitatively.
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