| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44721 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44743
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5017
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M35-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M47-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-33
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 |
| filingDate |
2000-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95f6c0d98164139dd43ecd74048d74f5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84546f92e4a00f0a472224c894428533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8483dd798399a69bc6dc7e7518a207e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_074666c8734170ad0318f40f3b3dc20b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2cab8ae897d8c20330c6c2a44cb4945
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0e264b268e68de0893c226c913653c7 |
| publicationDate |
2005-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1200821-A4 |
| titleOfInvention |
METHOD AND DEVICE FOR DETECTING ENZYMATIC ACTIVITY USING A MOLECULE THAT CHANGES ITS ELECTROPHORETIC MOBILITY |
| abstract |
The activity of intracellular chemical reactions of molecules is measured by the use of fluorescently labeled substrate molecules (114) that undergo a change in electrophoretic mobility upon chemical reaction such as that catalyzed by an enzyme (112). Specificity is achieved by using labeled substrate molecules (114) that can be acted upon only by specific enzymes (112). Thus, the activity of a specific enzyme (112) or class of enzymes can be determined. Measurements are made with the intracellular presence of such substrate molecules (114), at some time of interest, typically after exposure of the cell (46) to a stimulus (118) that activates a particular enzymatic pathway. To ensure accuracy, measurements must be made in a timely manner so as to minimize chemical reactions occurring subsequent to the time of interest. Fast controllable laser lysis is used to obtain the contents of a single cell (46) into which reporter substrate molecules (114) have been introduced. The cell contents are then subjected to capillary eletrophoresis (22) and enzymatic activity is determined by comparing amounts of substrate molecules (114) to amounts of enzymatically altered substrate molecules (128) which are separated by the electrophoresis and identified by the presence of a fluorescent label. |
| priorityDate |
1999-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |