http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1188838-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_add0d14b9a939865f2157689513b2342 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0024 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P35-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-02 |
| filingDate | 2000-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4e028115647195dc749ebc1b7766c12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_867a101e7557f8c98130648517e05223 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1078039738795e083ae947959ae0fe5f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36588cf19ee08b5577234d2de5e7fa20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42424649b1e2ee0b91a43c0c6d141318 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b580f3e988838e3bdb7b3fcdc1c8f068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_72d04b004765d2b2e00c1b52a5e6576b |
| publicationDate | 2002-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1188838-A1 |
| titleOfInvention | Method for producing 7-aminodesacetoxycephalosporanic acid (7-adca) |
| abstract | A method of obtaining 7-aminodesacetoxycephalosporanic acidn(7-ADCA). The method is based on the use of desacetoxycephalosporinnC (DAOC) produced directly by fermentation ofnAcremonium chrysogenum (also called Cephalosporium acremonium)nand catalysed by the enzymatic activities D-amino-acid oxidasen(DAO) and glutaryl-7-ACA acylase (GLA). The method of productionnof DAOC by fermentation of A. chrysogenum is based on inactivationnof the cefEF gene and subsequent expression of the cefE gene givingnrise to the strain A. chrysogenum ▵cefEF-cefE. The invention includesnthe following sections: (I) inactivation of the cefEF gene of A.nchrysogenum, which codes for the enzymatic activitiesndesacetoxycephalosporin C synthase (DAOCS or expandase) andndesacetylcephalosporin C synthase (DACS or hydroxylase), (II)nexpression of the cefE gene, which codes for the enzymatic activitynDAOCS, derived from an actinomycete in the transformant of A.nchrysogenum inactivated in the cefEF gene, (III) development of anmethod of fermentation of the recombinant strain for the production ofnDAOC and (IV) enzymatic conversion of DAOC to 7-ADCAncatalysed by the enzymes DAO and GLA. Alternatively, it would benpossible to produce 7-ADCA directly by fermentation of A.nchrysogenum by combined expression of the genes that code for DAOnand GLA in the DAOC-producing strain A. chrysogenum ▵cefEF-cefEnor by expression of a gene that codes for the enzyme cephalosporinnacylase in A. chrysogenum ▵cefEF-cefE. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012032040-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6642020-B2 |
| priorityDate | 1999-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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