| abstract |
A method for automated image analysis (Fig. 2, 42) of a biological specimen by histological reconstruction, provided is an automated cell image method for analyzing a biological specimen that has consecutively been stained by either an in situ hybridization (ISH) method, or an immunohistochemistry (IHC) method or a nucleic acid stain, and counterstained. The method couples composite images in an automated manner for processing and analysis to identify structure in tissue that cannot be captured in a single field of view image (Fig. 2, 44) or a single staining technique, the disclosure provides a method for histological reconstruction to analyze many fields of view on potentially many slides (Fig. 2, 37) simultaneously. |