| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c481903f97d0ab5938416073b384054 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-035
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-35
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-75 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate |
2000-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2005-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54c7fd1716aa9ca1c53e751fce89db5d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea469b10b59405300d81b236629f6194
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b736744f923a3104c4f8e5fe02e9428
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f5518bd6ca636961f1bf761e30ab5dc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2ab2ddeb9f2678de320e61f38d6bbe9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_798dc11613a63a83f448d52b06f9a56d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7018963c7ff240381f99dafd6765235 |
| publicationDate |
2005-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1173592-B1 |
| titleOfInvention |
Microbial protein expression system |
| abstract |
The present invention provides bacterial strains for secretion of soluble biologically active recombinant heterologous proteins into periplasm or on a surface/into a cultivation medium of bacteria. The invention exploits the secretion system of Gram-negative bacteria including periplasmic chaperones and usher/secretin proteins. For accomplishing the aim, the bacterial strains simultaneously express the fusion protein (signal peptide)-(mature hecterologous protein)-(subunit of a bacterial surface structure, Caf1), periplasmic chaperone specific for the subunit, and outer membrane usher/secretin protein specific for the subunit. Secretion of fusion proteins: (signal peptide of Caf1)-(mature human IL-1beta)-(mature Caf1), (signal peptide of Caf1)-(mature human GM-CSF)-(mature Caf1), and (signal peptide of Caf1)-(mature human IL-1ra)-(mature Caf1) that were expressed in Escherichia coli simultaneously with the periplasmic chaperone Caf1M and the usher/secretin protein Caf1A are examples of the use of the invention. |
| priorityDate |
1999-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |