| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69ece09bafee7e0c49d1f7951a3faa2c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21069
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6459
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-005 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
| filingDate |
2000-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2006-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d3b5e7da9ff3bd398ab8c043bec3f49
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e197292bb3e2ed5cab35bcdfa6e176c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba86e0067e28aca5ee945a674601225a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_541e78580ad01d4e6b1b84a818d731ae |
| publicationDate |
2006-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1171615-B1 |
| titleOfInvention |
Cell culture process for glycoproteins |
| abstract |
A glycoprotein is produced by a process comprising culturing mammalian host cells expressing nucleic acid encoding said glycoprotein in the presence of (a) a factor that modifies growth state in a cell culture, (b) a divalent metal cation that can adopt and prefers an octahedral coordination geometry, and/or (c) a plasma component. In this process, the occupancy of an N-linked glycosylation site occupied only in a fraction of a glycoprotein is enhanced. Such culturing is preferably carried out at a temperature of between about 30° C. and 35° C. and/or in the presence of up to about 2 mM of a butyrate salt and/or in the presence of a cell-cycle inhibitor. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1757700-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1757700-A3 |
| priorityDate |
1999-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |