http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1159414-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c5772effcbbda03d48ed9a7bbc0aad7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_226abe1c3cc289e9c0ce2fefe10ac6eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c8cbbf2290b3f3a9988da29598baa2c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e232689f4a1fc9ec99d4344c059b061b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2000-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9958db4238e34e8901545854acaefa19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97b78ac5ea0a87fe4f15ed0ab8702658 |
| publicationDate | 2001-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1159414-A1 |
| titleOfInvention | Process for enhancing electric field-mediated delivery of biological materials into cells |
| abstract | An object of the invention is to provide simple, nontoxic, and pharmaceutically defined methods for genetic modification of cells and tissues which enable development of a variety of molecular medicines. The invention herein is directed to a method for improving the transfer efficiency of biological material into cells and decreasing the frequency of cell death, in vitro, in vivo, and ex vivo. Specifically, the invention involves the synergistic employment of biopolymer binding reagents, e.g. polynucleotide binding reagents, in combination with electroporation techniques to enhance the efficiency of polynucleotide transfer to the cells of interest and to decrease the frequency of cell death of these cells. The improved electroporation-based gene therapies find particular utility in the transfer of genetic material into cells and has broad applicability for a wide range of gene therapy and polynucleotide vaccine applications. The improved methods of the present invention are useful with both ex vivo and in vivo delivery systems. In addition, the invention herein is not limited to the transport of polynucleotides but rather, is also applicable to the transport of other biopolymers, such as proteins and antigens across cell membranes. |
| priorityDate | 1999-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 745.