| abstract |
An assay method which involves: contacting a sample with a binding partner which bindsnspecifically to a first substrate molecule which may be in the sample but not to a second substratenmolecule which may be in the sample; and detecting a response indicative of binding, or of thenextent of binding, between the first substrate and the binding partner, characterised in that thenbinding partner includes a metal ion that is required for binding between the binding partner andnthe substrate or product and that the response detection is performed without separating thenbound first substrate from the unbound first substrate. |