http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1147230-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c32775641a2462e0c791af0b9b077e08 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
filingDate | 1999-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f57344020b2033feca1bb70b42916bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eac3a6b950c4ae78243941e1f83bab87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19cdabad0e743b86af5bfe13c5958734 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0cc8016592b7be556ffb1ea9b45485ea |
publicationDate | 2001-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1147230-A1 |
titleOfInvention | Method for controlling the extension of an oligonucleotide |
abstract | The present invention relates to a method for selectively extending an oligonucleotide primer along a specific target polynucleotide sequence in a mixture of polynucleotides. A combination is provided comprising the mixture, an oligonucleotide primer having a modification, and a binding substance for the modification wherein the binding substance binds to the oligonucleotide and prevents the extension of the oligonucleotide along the target polynucleotide sequence. The temperature of the combination is adjusted to a level sufficient to irreversibly denature the binding substance and permit the extension of the oligonucleotide primer along the specific target polynucleotide sequence. The invention has particular application in the amplification of nucleic acids. Also disclosed are kits for carrying out a method in accordance with the present invention. |
priorityDate | 1999-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 298.