| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad2cd796f8c4dcb425ddcc68707d3444 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-978
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-829 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-05 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 |
| filingDate |
1997-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6cca9b6ff9d0c22be1819b839acc6ea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d89a3f927705132fe953bd0762654a5b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_160992d5abe75af8fd138745077a279f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffed8d8084d654ad644e020fbaa2f693 |
| publicationDate |
2001-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1132467-A2 |
| titleOfInvention |
Novel creatine amidinohydrolase, production thereof and use thereof |
| abstract |
A creatine amidinohydrolase having the following physicochemical properties: Action: catalyzing the following reaction; creatine + H2O -> sarcosine + urea Optimum temperature: about 40 - 50 DEG C Optimum pH: pH about 8.0 - 9.0 Heat stability: not more than about 50 DEG C (pH 7.5, 30 min) Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5 - 10.0 mM Molecular weight: about 43,000 (SDS-PAGE) Isoelectric point: about 3.5, a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor. According to the present invention, a creatine amidinohydrolase having a smaller Km value than that of the conventionally known creatine amidinohydrolase can be produced in an industrially large amount, and can be used as a routine reagent for clinical tests for determining creatine and creatinine in biological samples. |
| priorityDate |
1996-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |