| abstract |
The amino acid sequence of native contortrostatin was used in a cloning strategy to obtain full-length cDNA and deduced amino acid sequnces for a contortrostatin precursor. The precursor includes pro-protein, metalloproteinase, and disintegrin (contortrostatin) regions of the multidomain protein. The sequences can be used to produce recombinant DNA molecules which code on expression for contortrostatin proteins, including biologically active variants and fragments. When formulated as a pharmaceutically acceptable composition, the proteins can be used to treat patients by inhibiting disease processes associated with an integrin binding to an integrin receptor. |