| abstract |
The present invention provides a method for purifying follicle stimulatingnhormone (FSH) from biological samples, for example, from human pituitary glands ornhuman postmenopausal urine, wherein the FSH is contaminated with other proteins,nby use of dye-ligand affinity chromatography (DAC). Depending on the startingnmaterial used and the initial purity of FSH in the starting material, additionalnpurification steps may be employed. These steps preferably involve the use ofnhydrophobic interaction chromatography. This process may be used to generatenaffinity pure FSH suitable for therapeutic applications. The methods of the inventionnprovide high purity FSH with high overall yield. A further advantage is the ability toneasily regenerate the chromatography media for re-use, thus providing added economynto the purification process. |