| abstract |
A method of amplifying a specific nucleic acid fornassay of the specific nucleic acid anticipated in ansample by an RNA amplification procedure which comprisesnforming a double-stranded DNA which contains sequencesncomplementary and homologous to the specific RNA sequencenand has a promoter sequence enabling transcription of thensequence by using the target RNA as the template andnforming by using an RNA polymerase an RNA transcriptnwhich acts as the template for formation of a new single-strandednDNA, wherein in the RNA amplification procedure,ninosine triphosphate is added in addition to adenosinentriphosphate, uridine triphosphate, cytidine triphosphatenand guanosine triphosphate to improve the efficiency ofnthe amplification reaction. |