http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1101093-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_29b015ec698ae9270d29530382bdc967 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-804 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-555 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-555 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 1999-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2006-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbe78fd736eb881549d070f25abd7fcf |
| publicationDate | 2006-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1101093-B1 |
| titleOfInvention | Test for oxidative stress using cell suspensions |
| abstract | This invention provides a method of measuring oxidative response of cells without recourse to preparation of cell culture. The process involves:1) preparing suspensions of cells from a living host in isotonic solutions,2) preparing samples of test materials in isotonic solution containing tagged choline,3) adding the cells suspension prepared in step 1 to the samples prepared in step 2,4) incubating the product of step 3 with shaking for 2-90 minutes,5) extracting and drying the lipid phase from the product of step 4, and6) subjecting the product of step 5 to a scintillation counter to measure choline which has been incorporated into phosphatidylcholine (PC). An increase in incorporation of choline into PC in the short term indicates oxidative stress or free radical induced damage. Because the method of the invention using the cell isolates does not require the expense of cell culture with concomitant expense and possibility of cell change, it is particularly useful for clinical evaluation. Furthermore, the ability to use erythrocytes for study of cellular response to oxidative stress makes it possible to test individual responses of patient using part of the blood sample drawn routinely for laboratory use. |
| priorityDate | 1998-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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