| abstract |
Hairpin-forming oligonucleotide probes and primers are triple-labelled with a pair of florophores, a shorter wavelength harvester and a longer wavelength emitter, and a quencher. When the probes and primers are stimulated by light that excites the harvester, opening causes and increase in fluorescence by the emitter, while fluorescence from the harvester is continually suppressed. The probes and primers may be used for detection of nucleic acid targets in assays, including amplification assays. Assay kits are provided. |