Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bcb146c68b67089013b4c7a614504254 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
1999-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43331e837ff1c9d36f15f2c785fffd0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98ab943f02dabed03e22b8f527975969 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7761452b4218d930e1a34dea733d853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c250b6206acde070b0b8bf64b7e721e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b898a5f7e85166ca728507dc136f4483 |
publicationDate |
2001-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-1064400-A1 |
titleOfInvention |
Nucleic acid detection |
abstract |
This invention discloses methods for detecting specific nucleic acid sequences, interrogating the identity of a specific base within a sequence, and assaying endonuclease and exonuclease activity. DNA or RNA probes are hybridized to target nucleic acid sequences. Probes that are complementary to the target sequence at each base are depolymerized, while probes which differ from the target at the interrogation position are not depolymerized. The nucleic acid detection systems utilize the pyrophosphorolysis reaction catalyzed by various polymerases to produce deoxyribonucleoside triphosphates or ribonucleside triphosphates. dNTPs are transformed to ATP by the action of NDPK. The ATP produced by these reactions is detected by luciferase or NADH based detection systems. |
priorityDate |
1998-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |