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filingDate 2000-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_444d9ea2ee4742954c1136c3112d145c
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publicationDate 2000-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1061369-A2
titleOfInvention Element, method and kit for the determination of an analyte in a fluid
abstract enthaltend in oder auf Material, das einen Flüssigkeitstransport zwischen Zonen ermöglicht,neine Probenauftragszone (1) und eine hierzu flußabwärts gelegene Nachweiszonen(4) sowie zwischen Probenauftragszone (1) und Nachweiszone (4) eine Zone (3) mit immobilisiertemnAnalyt oder Analytanalogon und flußaufwärts der Zone (3) mit immobilisiertem Analyt oder Analytanalogon durchnFlüssigkeit ablösbar imprägniertes Konjugat 1 aus einem mit dem zu bestimmendennAnalyt zu einer spezifischen Bindungsreaktion fähigen bioaffinen Bindungspartner 1 undneiner detektierbaren Markierung 1, ndadurch gekennzeichnet, daß die detektierbare Markierung 1 ein niedermolekulares organisches Molekül ist und flußaufwärts der Zone (3) mit immobilisiertem Analyt oder Analytanalogon ebenfallsndurch Flüssigkeit ablösbares, universelles Konjugat 2 aus einem mit der detektierbarennMarkierung 1 zu einer spezifischen Bindungsreaktion fähigen bioaffinen Bindungspartnern2 und einer visuell erkennbaren Markierung 2 vorhanden ist. The invention relates to an element for determining an analyte in a liquid by means of a specific binding reaction of two bioaffine binding partners Containing a sample application zone (1) and a detection zone (4) downstream of it in or on material that enables liquid transport between zones and between the sample application zone (1) and detection zone (4) a zone (3) with immobilized analyte or analyte analog and upstream of zone (3) with immobilized analyte or analyte analog, liquid-releasably impregnated conjugate 1 consisting of a bioaffine binding partner 1 capable of a specific binding reaction with the analyte to be determined and a detectable label 1, characterized in that the detectable label 1 is a low molecular weight organic molecule and upstream of zone (3) with immobilized analyte or analyte analog there is also liquid-detachable, universal conjugate 2 consisting of a bioaffine binding partner 2 capable of a specific binding reaction with the detectable marker 1 and a visually recognizable marker 2. n n n The invention also relates to the use of an element according to the invention for determining an analyte and a kit for determining an analyte containing an element according to the invention and eluents.
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