http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1051520-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b8be6a6c990669567cc455d32b00535e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 1999-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32a6cf004113f4e2ce4cb3eaefbcd7a6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa1dc6c02ac9f19f516b1f8860587da9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e02e09b51f01b9ed778b192c6ea5acc |
| publicationDate | 2000-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1051520-A1 |
| titleOfInvention | Improved method for the isolation of nucleic acid |
| abstract | The present invention relates to a method for isolating nucleic acid from a sample. Known methods for the isolation of nucleic acid from these materials may comprise the use of a chaotropic substance, for example to lyse cells present in the starting material, and free the nucleic acid therefrom. When a nucleic acid containing material is treated with a chaotrope and contacted with a nucleic acid binding solid phase, the nucleic acid will bind to the solid phase in the presence of the chaotrope and the solid phase with the nucleic acid bound thereto can thus be separated from the remainder of the sample. The method of the present invention for the isolation of nucleic acid from nucleic acid containing starting material is a method wherein the starting material is contacted with a chaotropic substance and, either simultaneously or subsequently, with a nucleic acid binding solid phase, where after the nucleic acid binding solid phase is subjected to a washing procedure and, optionally, the nucleic acid is eluted from the nucleic acid binding solid phase, characterized in that the washing procedure comprises the steps of washing the solid phase with subsequently one ore more high salt buffer solution(s), optionally an alcoholic solution and a low salt buffer solution. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104603267-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104603267-B |
| priorityDate | 1998-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.