http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1046710-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ea111b63b97ac4b8bc9897778000bc78 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-203 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-002 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4702 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 1999-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d3e02cb34c9a28cd78f25057de5486f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4d81c1e4fcd392a13de8baffae06e16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7034608e6a4f3c1521c29a6e835580d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bee4fa4c68d9183ab64e5454af7abcf |
| publicationDate | 2000-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1046710-A1 |
| titleOfInvention | Promoter-transactivator system for inducible high-level mammalian gene expression with the option of cell growth control |
| abstract | Promoter-transactivator system for inducible high-level expression of mammalian genes, and optionally for control of cell growth, comprises: (i) a promoter construct (IRFE promoter); and (ii) transactivator construct encoding a fusion protein of IRF-1 (interferon regulating factor-1) and the estrogen receptor (ER). IRFE has the structure [MPSV-E]-[IRF-1 binding site]-[CMV]-DNA where MPSV-E indicates the myeloproliferative sarcoma virus enhancer repeat (270 bp, sequence reproduced); IRF-1 is a 70 bp sequence reproduced, and CMV is the cytomegalovirus minimal promoter (74 bp sequence reproduced) or their functionally equivalent variants with one or more nucleotides substituted, inserted or deleted. Independent claims are also included for the following: (1) an expression vector containing these promoter and/or transactivator constructs; (2) mammalian cells transformed or transfected with the vectors of (a); and (3) a method for inducing high level expression of mammalian genes with the option of controlling cell growth. MECHANISM OF ACTION : Transcription regulator. Increased transcription results from binding of the IRFE promoter to the transactivator, which is activated by estradiol or other ER ligands (these compounds displace the heat-shock protein 90 which normally binds to the IRF-1/ER fusion, preventing its activation). |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02068614-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02068614-A3 |
| priorityDate | 1999-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.