http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1046709-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b828305495e1cdb63947a3a18aa633d1 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P5-007 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 |
filingDate | 1999-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39360cd730590bc1a83e2ba72a37ae60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b22f22ecf53abb9e0fb009db2e459352 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_355e803163a70be3fa64eba91793656e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d809db8af6791af58d94c8f79ed3691b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bdd8ce91b3e17652040b356095feb2f |
publicationDate | 2000-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1046709-A1 |
titleOfInvention | Isopentenyl diphosphate isomerase from Hevea Brasiliensis and rubber producing method using the same |
abstract | The present invention cloned a cDNA clone encoding isopentenyl diphosphaten(hereafter "IPP") isomerase (EC 5.3.3.2) from a cDNA library of Hevea brasiliensis nlatex. The clone has a continuous open reading frame encoding a peptide of 234namino acids with a predicted molecular mass of 26.7 kDa. The deduced protein isnacidic with an isoelectric point of 4.7 and shows high sequence identity with other IPPnisomerases. The recombinant protein expressed in Escherichia coli showed IPPnisomerase activity. In vitro rubber biosynthesis assays using washed rubber particlen(WRP) deprived of initiating allylic diphosphates were performed with the addition ofnIPP isomerase in the reaction mixture. Results revealed that the recombinant IPPnisomerase is catalytically active in catalyzing the conversion of IPP to DMAPP, a keynactivation step of the basic five-carbon isoprene unit in rubber biosynthesis.nSouthern analysis indicated that the IPP isomerase is encoded by two genes in Hevea nrubber tree. In Northern blot analysis, two different sizes of transcripts (1.2 and 0.6nkb) were detected from leaf tissues while only one hybridizing band (1.0 kb) wasndetected from latex. Analyses of RNA extracted from extruded latex and leaf tissuesnof the trees wounded with nails showed that wounding did not change the transcriptnlevel of IPP isomerase. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103153044-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016178553-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103153044-B |
priorityDate | 1999-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 393.