| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5318709ebf1fa2152a71cb8513c2dd73 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-315
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-46 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-315 |
| filingDate |
2000-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a768c244659252cbea9f12484658ff54 |
| publicationDate |
2000-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1035202-A2 |
| titleOfInvention |
A cloning method for DNA fragments using arbitrarily primed PCR |
| abstract |
A method of cloning DNA fragments is disclosed comprising the steps of: (a)ncleaving a vector DNA of plasmid origin with a restriction enzyme, (b)ndephosphorylating an end of thus obtained cleaved DNAs, (c) separately obtaining anmixture of DNA fragments by cleaving a chromosomal DNA of a given organismnwith the same restriction enzyme as the aforementioned restriction enzyme, (d)nobtaining a mixture of ligated DNAs through ligation using the dephosphorylatednDNAs obtained in step (b) and the mixture of DNA fragments obtained in step (c),n(e) amplifying the ligated DNAs by PCR with arbitrary primers using the obtainednmixture of ligated DNAs as templates, and (f) introducing into a competent cell ancloning vector into which is incorporated a PCR product thus obtained. |
| priorityDate |
1999-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |