| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a546384a5eedf2f5c8026fc8dd0ac6d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_59e1d955a7b1560dc68cf7fd800e8fac |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2799-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-715 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-715
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 |
| filingDate |
1998-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eac112b8dd41051fc4d5609ee62bb104
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_175cb91f84ccd3ff2d663faf28d92d82 |
| publicationDate |
2000-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1033374-A1 |
| titleOfInvention |
Signal sequence trapping method |
| abstract |
A DNA encoding human mpI lacking the secretory ability,nprepared by deleting the secretory signal of homeostaticallynactive mpl and the major part of the extracellular regionnthereof. This DNA is ligated with a test cDNA, a cDNA encodingna known secretory protein and another cDNA encoding the samenprotein from which the secretory signal region is removed, eachnof the resulting chimeric genes is expressed in cells to examinencell proliferation capability. Cell proliferation is detectednin the cells containing the cDNA encoding the known secretorynprotein as a test cDNA, whereas no cell proliferation isndetected for the cDNA encoding the same protein from which thensecretory signal region is removed. Thus, cDNAs encodingnsecretory proteins including type I membrane proteins and typenII membrane proteins can be detected and isolated bynconstructing a cDNA library and screening it by the abovenmethod. |
| priorityDate |
1997-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |