| abstract |
A process is provided for the preparation of albumin which has extremely low levels ofnor is essentially free of colorants, metal ions, human proteins, host proteins, fragmentsnof albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated,nrelatively high in free thiol and with an intact C-terminus. The processncomprises passing albumin (preferably expressed and secreted by transformed yeast)nthrough positive mode cation exchange and then positive mode anion exchangenchromatography. Other steps may also be employed, for example ultrafiltration, gelnpermeation chromatography, affinity chromatography binding the albumin (for examplenusing blue dyes) and affinity chromatography binding contaminants (for example usingnan aminophenylboronic acid resin). Elution of albumin, with a compound having affinitynfor albumin, from a material having no specific affinity for albumin is also disclosed, asnis removal of ammonium ions with a counter-ion. Albumin of high purity is preferred bynthe above process. |