abstract |
The present invention relates to an oligonucleotidenhaving a novel structure and a method of synthesizing nucleicnacid by using the same as a primer. This oligonucleotide isnprovided at the 5'-side of the primer with a nucleotide sequencensubstantially the same as a region synthesized with this primernas the origin of synthesis. The present invention realizesnsynthesis of nucleic acid based on an isothermal reaction withna simple constitution of reagents. Further, the presentninvention provides a method of synthesizing highly specificnnucleic acid on the basis of this method of synthesizing nucleicnacid. |