http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1016731-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_379c7f27255c8dc9f1098244a3e60689 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6862 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6823 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6862 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1999-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de76e2f884bcde66e5b833b08c20601e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e7481b40f7a9e92c12dad0a5be540f0 |
| publicationDate | 2000-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1016731-A1 |
| titleOfInvention | Probes for detecting target nucleic acid, method of detecting target nucleic acid, and solid phase for detecting target nucleic acid and process for producing the same |
| abstract | This invention relates to a pair of probes for thendetection of a target nucleic acid, said pair of probesncomprising (1) probe 1 having base sequence B1ncomplementary to A1 between two specific sequentialnbase sequences A1 and A2 of the nucleic acid, whereinn(a) the 5'-terminus of base sequence B1 isnphosphorylated and (b) a first solid phase immobilizingnpart is bound to the 3'-terminus of base sequence B1,nand (2) probe 2 having base sequence B2 complementarynto A2 between base sequences A1 and A2, wherein (a) then5'-terminus of base sequence B2 is bound to one end ofna cleavage part and (b) a second solid phasenimmobilizing part is bound to the other part of thencleavage part. When the target nucleic acid isnhybridized above the solid phase of the invention wherensaid probes are immobilized on its surface, the probesnon the solid phase occupy spatial positions beneficialnto the formation of a hybrid and thus forms the hybridnefficiently. Therefore, probe 1 and probe 2 arenefficiently ligated by ligase reaction. Furthermore,nafter the target nucleic acid is removed from thenhybrid, only probe 2 ligated as described above will benable to exist on the solid phase through cleavagenreaction of the cleavage part. Therefore, after thenfree probe 2 has been removed by washing, it will nbecome possible to detect the presence of probe 2 onnthe solid phase ligated as described above, with highnsensitivity and high recognition. This will enablendetection of the presence of a target nucleic acid withnhigher sensitivity and higher recognition as comparednto methods in the prior art. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005118871-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2936246-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006078289-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2005325273-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006104979-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006104979-A2 |
| priorityDate | 1998-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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